Peptide Dosage Calculator

Correct Peptide Reconstitution

Peptides are delicate biomolecules that require precise handling and storage to maintain their integrity and effectiveness. Our peptides are delivered as lyophilized (freeze-dried) powders, which offer stability against short-term temperature fluctuations during transit. To maximize their shelf life and ensure consistent results, proper storage and reconstitution methods must be followed.

Storage and Handling Best Practices

Before Reconstitution: Store lyophilized peptides in a dry, cool place, preferably at−20°C to −80°C for long-term preservation. This prevents degradation and contamination.

After Reconstitution: Once peptides are reconstituted, they become more susceptible to degradation. Store solutions at 4°C for short-term use (a few days) or freeze aliquots at −20°C for longer periods. Avoid repeated freeze-thaw cycles, as these can reduce the peptide's bioactivity.

How to Reconstitute Peptides Properly

Choose the Right Solvent: The solvent choice depends on the peptide's solubility:

• Bacteriostatic water — Suitable for many peptides but may not dissolve hydrophobic peptides completely.
• Acetic acid (0.1%) or dilute HCl — Can be used sparingly for highly hydrophobic peptides.
• Other buffers — Used when specific pH conditions are required.

Gently Add the Solvent: Add solvent slowly to the vial (dropwise) to avoid foaming. Mix by gently swirling or tilting; do not vortex or shake aggressively.

Check for Clarity: Ensure the solution is clear and free of undissolved particles. If needed, sonication (brief exposure to ultrasonic waves) can help dissolve stubborn peptides.

Common Mistakes to Avoid

• Using tap or non-sterile water, which may introduce contaminants.
• Shaking the vial aggressively, which can degrade the peptide.
• Allowing the reconstituted solution to sit at room temperature for extended periods.
• Not aliquoting solutions for storage, leading to repeated freeze-thaw cycles.