VIP — Vasoactive Intestinal Peptide
Dosing & reconstitution protocol
Quick Reference
Overview
Vasoactive Intestinal Peptide (VIP) is a 28-amino acid neuropeptide that acts on VPAC1 and VPAC2 receptors throughout the central nervous system, gastrointestinal tract, and pulmonary tissue. Research applications span immune modulation, pulmonary vasodilation (CIPA/PAH models), neuroinflammation, circadian rhythm regulation, and gut-brain axis studies. VIP has a very short plasma half-life (~1–2 minutes via IV); subcutaneous delivery slows absorption and extends effective exposure. Intranasal delivery is used in neurological research for direct CNS access.
Dosing & Reconstitution Guide
| Phase | Daily Dose | Units | Volume |
|---|---|---|---|
| Weeks 1–2 | 25 mcg per dose | Per reconstitution | Per concentration |
| Weeks 3–6 | 50 mcg per dose | Per reconstitution | Per concentration |
| Weeks 7–12 (optional) | 100 mcg per dose | Per reconstitution | Per concentration |
Reconstitution Instructions
Reconstitute the lyophilised VIP vial with 1.0–2.0 mL bacteriostatic water, depending on desired concentration.
Inject the water slowly down the vial wall; do not shake — VIP is sensitive to mechanical agitation.
Gently swirl until fully dissolved; solution should be clear and colourless.
Label with reconstitution date; refrigerate at 2–8 °C and use within 14 days.
Due to VIP's short stability window, prepare smaller reconstitution volumes (1 mL) for higher-frequency protocols.
This guide is for educational purposes only and is not medical advice. For research use only. Not for human consumption.
Supplies Needed
- ✓VIP Vials: 2–4 vials
- ✓Insulin Syringes (U-100): 56 syringes
- ✓Bacteriostatic Water (10 mL): 1 bottle
- ✓Nasal Atomiser Device (if intranasal route): 1
- ✓Alcohol Swabs: 60 swabs
Dosing Protocol
Schedule
Administer once or twice daily via subcutaneous injection. For neurological research, intranasal delivery (IN) is performed using a nasal atomiser device, typically 50–100 mcg per nostril per dose.
Cycle Length
4–12 weeks depending on the research endpoint. Immune and pulmonary protocols typically use shorter cycles (4–6 weeks); neurological studies may extend to 8–12 weeks.
Stability
VIP is susceptible to oxidation and enzymatic degradation. Prepare fresh reconstitutions every 10–14 days. Avoid repeated freeze-thaw cycles of reconstituted solution.
Storage & Handling
- ✓Lyophilized: Store at −20 °C (−4 °F) in dark, dry conditions; VIP is more sensitive to temperature than most peptides.
- ✓Reconstituted: Refrigerate at 2–8 °C; use within 14 days (shorter stability than many peptides).
- ✓Do not freeze reconstituted VIP solution — peptide aggregation is irreversible.
- ✓Minimise air headspace in the vial after each draw to reduce oxidative degradation.
- ✓Discard if solution is not clear or if particulates are visible.
Important Notes
- ✓All products sold by JA Performance Peptides are intended strictly for laboratory research purposes and are not approved for human consumption, veterinary use, or therapeutic application. Not for human use.
- ✓VIP is a short-half-life peptide; timing of sampling relative to injection is critical for pharmacokinetic research designs.
- ✓Use a new sterile syringe for each injection; dispose in a sharps container.
- ✓For intranasal administration, prime the atomiser device before first use and calibrate the spray volume per protocol.
- ✓Rotate subcutaneous injection sites to avoid localised hypoperfusion from the vasodilatory effects of VIP.
- ✓Document dose, route, time, and any observed physiological responses at each administration point.