Metabolic Core Stack — NAD+ + MOTS-C + 5-Amino-1MQ
Cellular energy & metabolic optimization stack protocol
Quick Reference
Overview
The Metabolic Core Stack combines three mechanistically distinct compounds targeting cellular energy production, mitochondrial health, and metabolic regulation from complementary upstream angles. NAD+ (nicotinamide adenine dinucleotide) is an essential coenzyme in the mitochondrial electron transport chain and a substrate for sirtuins (SIRT1–SIRT7) and PARPs — enzymes central to energy metabolism, DNA repair, and aging research. MOTS-C is a mitochondrial-derived peptide that activates AMPK, regulates glucose uptake and mitochondrial biogenesis, and enhances metabolic flexibility, with particular relevance in exercise physiology and insulin sensitivity models. 5-Amino-1MQ is a small-molecule NNMT (nicotinamide N-methyltransferase) inhibitor that increases intracellular NAD+ availability, promotes fat cell browning through increased SAM/SAH ratios, and supports energy expenditure pathways. Note: reconstitute NAD+ with sterile water (not bacteriostatic water) to avoid high benzyl alcohol load at therapeutic doses.
Dosing & Reconstitution Guide
| Phase | Daily Dose | Units | Volume |
|---|---|---|---|
| NAD+ (daily) | 50 mg | 50 units (at 0.5 mg/mL per label) | 0.25 mL at 2 mg/mL |
| NAD+ (advanced) | 100 mg | 100 units | 0.50 mL at 2 mg/mL |
| MOTS-C (2–3×/week) | 5–10 mg per injection | 50–100 units (at 1 mg/mL) | 0.50–1.0 mL |
| 5-Amino-1MQ (daily) | 50–100 mg | Per vial concentration label | Per vial concentration label |
Reconstitution Instructions
Allow all three vials to reach room temperature before handling.
Reconstitute NAD+: draw 2.0 mL sterile water (not bacteriostatic water) with a sterile syringe; inject slowly down the vial wall and swirl until fully dissolved.
Reconstitute MOTS-C: draw 1.0 mL bacteriostatic water with a fresh syringe; inject slowly and swirl gently.
Reconstitute 5-Amino-1MQ: draw 1.0 mL bacteriostatic water with a fresh syringe; inject slowly and swirl until dissolved.
Label each vial with the compound name and reconstitution date; refrigerate at 2–8 °C, protected from light. Use within 28 days.
This guide is for educational purposes only and is not medical advice. For research use only. Not for human consumption.
Supplies Needed
- ✓NAD+ Vials (per label mg): 4–8 vials depending on vial size
- ✓MOTS-C Vials: 2–4 vials
- ✓5-Amino-1MQ Vials: 4–6 vials
- ✓Insulin Syringes (U-100): 150 syringes
- ✓Sterile Water (10 mL) — for NAD+: 2 bottles
- ✓Bacteriostatic Water (10 mL): 1 bottle
- ✓Alcohol Swabs: 300 swabs
Dosing Protocol
NAD+ Schedule
Inject 50–100 mg subcutaneously daily or every other day. Start at 50 mg and titrate based on tolerance — transient flushing or warmth at the injection site is common at higher doses. Subcutaneous administration provides slower, more comfortable absorption than IV.
MOTS-C Schedule
Inject 5–10 mg subcutaneously 2–3 times per week (e.g., Monday / Wednesday / Friday). In exercise-related research protocols, administer 30–60 minutes before physical activity to examine acute metabolic responses.
5-Amino-1MQ Schedule
Inject once daily subcutaneously at a consistent time. Daily dosing maintains sustained NNMT inhibition and elevated intracellular NAD+ throughout the research period.
Cycle Structure
Run all three compounds concurrently for 8–12 weeks, followed by a 4-week off period. NAD+ may be continued at lower frequency (2–3×/week) during rest periods to maintain baseline coenzyme levels.
Storage & Handling
- ✓Lyophilized: Store all vials at −20 °C (−4 °F) in dry, dark conditions.
- ✓Reconstituted: Refrigerate at 2–8 °C (35.6–46.4 °F); keep each vial clearly labelled and separate.
- ✓NAD+ must be reconstituted with sterile water — not bacteriostatic water — to avoid accumulating benzyl alcohol at high doses.
- ✓Do not freeze reconstituted solutions.
- ✓Allow vials to reach room temperature before opening to reduce condensation.
- ✓Discard if any solution becomes cloudy or develops visible particulates.
Important Notes
- ✓All products sold by JA Performance Peptides are intended strictly for laboratory research purposes and are not approved for human consumption, veterinary use, or therapeutic application. Not for human use.
- ✓Keep all compounds in separate, clearly labelled vials — do not mix in the same syringe.
- ✓Use sterile (not bacteriostatic) water for NAD+ reconstitution to minimise benzyl alcohol load in high-dose protocols.
- ✓NAD+ at 100 mg+ may cause transient flushing, warmth, or mild nausea — reduce dose if symptoms persist.
- ✓MOTS-C may produce mild injection site warmth; this is an expected response.
- ✓Document daily doses, injection sites, and timing for each compound separately.